SDS Volume

Volume 20. Halogenated Benzenes, Toluenes and Phenols with Water, p 232

Source Publication

Blackman, G. E.; Park, M. H.; Garton, G.; Arch. Biochem. Biophys. 1955, 54(1), 55-71.

Conditions:

Room Temp: 25 °C
Acidity: 5.1

Compiler:

F.W. Getzen

Methodology:

Equilibrium was established by mixing the solute with distilled water buffered with $\ce{KH2PO4}$ at a pH of 5.1 in a glass-stoppered bottle suspended in a thermostat bath at 25 °C. The sample was shaken periodically for two days with appropriate adjustment of pH to 5.1. After the pH became stabilized (usually about one week), the sample was equilibrated for an additional two weeks to assure saturation. Concentration was determined either directly by spectrophotometric techniques or by a colorimetric method based upon that given by [1] in which suitable colorizing agents [2,3] were added either to the saturated solution or to an appropriately diluted solution to yield an optical density proportional to the solute concentration. Concentration was obtained as the average of three replicate measurements.

Sample Information:

1. Probably a commercial reagent.
2. Distilled water.

Measurement Errors:

Mass concentration (m/v) (1): <5% (evaluated on the basis of the reported results of the two analytical techniques.

Methodology Citations

¹ Snell, F. D.; Snell, C. T. 'Colorimetric Methods of Analysis', Vol. II; Chapman and Hall: London, England, 1936.
² Folin, O.; Denis, W.; J. Biol. Chem. 1912, 12, 239.
³ Folin, O.; Ciocalteu, V.; J. Biol. Chem. 1927, 73, 627.