P.A. Meyers, David G. Shaw Solubility, Solubility data series Dodecane solutions were prepared in a chemically defined solution resembling dilute seawater. In addition to the mineral salts and vitamins reported [1], the solution contained 1 mg/L arginine and 10 g/L NaCl. After sterilization (121 °C for 30 min), 0.8 µL of $\ce{^{14}C}$-dodecane was sprayed on to the medium surface to form a thin film. Gentle subsurface agitation was induced by a 3 in magnet rotating at 60 rev/min against the inside carboy wall at midlevel. The subsurface dodecane concentration was determined from the radioactivity of samples collected from a port at the bottom of the carboy. The system was stirred for 1 week and then allowed to stand. Equilibrium appeared to have been achieved within eight weeks. The International Union of Pure and Applied Chemistry Button, D. K.; Limnol. Oceanogr. 1969, 14, 95-100. paper https://doi.org/10.4319/lo.1969.14.1.0095 Button, D. K.; Geochim. Cosmochim. Acta 1976, 40, 435-440. paper https://doi.org/10.1016/0016-7037(76)90008-9 112-40-3 1 C12H26 InChI=1S/C12H26/c1-3-5-7-9-11-12-10-8-6-4-2/h3-12H2,1-2H3 SNRUBQQJIBEYMU-UHFFFAOYSA-N 170.335 Dodecane mix-007 2 NaH2PO4.H2O}$, $\ce{(NH4)SO4}$, $\ce{KCl}$, $\ce{MgSO4}$, $\ce{CaCl2.2H2O}$, $\ce{ZnSO4.7H2O}$, $\ce{Fe(NH4)2(SO4)2.6H2O}$, $\ce{CuSO4.5H2O}$, $\ce{MnSO4.7H2O}$, $\ce{CoSO4.7H2O}$, $\ce{MoO3 0.0 Salt mixture [1] 7732-18-5 3 H2O InChI=1S/H2O/h1H2 XLYOFNOQVPJJNP-UHFFFAOYSA-N 18.0153 Water Dodecane with Salt mixture and Water